Standardisation of Artemisia annua using Reversed Phase High Performance Liquid Chromatography (RP-HPLC)

by Amos Abolaji O.(1), Ubana Eteng M.(2), Patrick Ebong E.(2), Andi Brisibe(3), Ahmed Shakil(4), Erun Shaista(4) and Choudhary Iqbal M.(4)


Artemisia annua L is a traditional Chinese medicine for the treatment of many disorders including drug resistant malaria. The active constituent against malaria, artemisinin, has been isolated and characterized by Chinese scientists. Artemisia annua is now being grown in malaria endemic countries like Nigeria. The aim of the present study was to quantify artemisinin content of Nigerian Grown Artemisia annua using a fast and reliable Reversed Phase-High Performance Liquid Chromatography (RP-HPLC) analytical technique since artemisinin content is affected by geographical and seasonal variations. HPLC conditions used for determination of the artemisinin content were established as follows; phenomenex luna column (5 μm; 250 mm × 4.6 mm) was employed with the mobile phase of acetic acid (% 0.1 v/v): acetonitrile: H2O (70:30) mixture at the flow rate of 1 ml/min. The good linearity of artemisinin was observed with y = 11714 × (r2 = 0.9989). Artemisinin was detected in our sample and was calculated to be 1.0975 %.
Keywords: Artemisia annua L., Artemisinin, HPLC, Standardisation


1 – Department of Biological Sciences, College of Science and Technology, Covenant University, P.M.B 1023 Canaan Land, Ota, Ogun State, Nigeria. +2348068614194
2 – Department of Biochemistry, College of Basic Medical Sciences, University of Calabar, P.M.B 1115, Calabar, Cross River State, Nigeria.
3 – Department of Genetics, Faculty of Science, University of Calabar, P.M.B 1115, Calabar, Cross River State, Nigeria.
4 – Industrial Analytical Centre, HEJ Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, 75270, Pakistan.

Prof Mbeh Eteng

Mbeh Eteng is a Professor of Biochemistry in the Faculty of University of Calabar. He holds a Ph.D from the University of Calabar (2000). He was Dean of Faculty of Basic Medical Science (2012-2016) and Chairman Committee of Deans (2014-2015). Member Governing Board UCTH, Calabar (2018 -till date). Fellow, Institute of Co-operate Administration. His current area of research is on malaria/prostrate hyperplaxia and medicinal plants research and use in management. He has successfully supervised 25 Ph.Ds.
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