INDUCTION OF MODEL TIME-RELATED AIRWAY INFLAMMATION IN WISTAR RATS WITH CRUDE EXTRACT OF PERIPLANETA AMERICANA by M. E. EKO, M. U. ETENG, D. N. OMOKARO AND I. B. UMOH
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ABSTRACT
Pulmonary inflammation in bronchial airways is an obvious hallmark in bronchial asthma. Asthma-like responses were induced in, wistar rats with crude extract of Periplaneta americana. Wistar rats were sensitized with the crude extract on day 0, and challenged twice with tne extract on days 14 and 21. Bronchoalveolar lavage fluid (BAL) extracted from crude extract-induced rats and Phosphate buffered saline (PBS)-induced control rats were examined at 12 hourly intervals up to 72 hours post induction. The crud,e.extract-induced rats displayed a high level of airway inflammation characteristic of cockroach-induced asthma compared with the PBS-induced rats: This involved increased total leukocyte counts, percentage neutrophils, lymphocytes and eosinophils at all time points examined. There were peak inflammation time of 24 hours for eosinophils and lymphocytes and 36 hours for neutrophils. The results showed evidence of late airway inflammation in BAL of P. americana’ crude extract-induced wistar rats comparable to airway inflammation of antigen-sensitized human asthmatics. These results are discussed together with the significance of wistar rat asthma model in the current herbal asthma therapy validation trials.
KEYWORDS: Inflammation, Asthma, Induction, P. americana, Rats.